Description: Cytochrome P450 Protocols Please note: this item is printed on demand and will take extra time before it can be dispatched to you (up to 20 working days). Author(s): Ian R. Phillips Format: Paperback Publisher: Humana Press Inc., United States Imprint: Humana Press Inc. ISBN-13: 9781617375729, 978-1617375729 Synopsis For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.
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Book Title: Cytochrome P450 Protocols
Number of Pages: 364 Pages
Publication Name: Cytochrome P450 Protocols
Language: English
Publisher: Humana Press Inc.
Item Height: 235 mm
Subject: Chemistry
Publication Year: 2010
Type: Textbook
Item Weight: 557 g
Author: Ian R. Phillips
Item Width: 155 mm
Series: Methods in Molecular Biology
Format: Paperback
