ECL Chemiluminescence Kit

Description: Description Storage and Handling Conditions Wet ice bag transportation; Stored at 4? in the dark; valid for 12 months. Assay Protocol 1. Preparation of hypersensitive ECL working solution: Mix the hypersensitive ECL A solution and hypersensitive ECL B solution in equal volumes, and store at 4? away from light. Preparation before use, please use within 2 days. 2. In Western blot experiment, after membrane was incubated with secondary antibody, wash several times with 1x PBS, and remove excess liquid. Add the mixed ECL working solution to cover the membrane incubate for 1-2 min. 3. Remove the membrane from ECL working liquid with forceps for imaging. Note: 1. High sensitive ECL A liquid and high sensitive ECL B liquid in the suction process, the pipette tips must be replaced. The cross contamination of liquid A and liquid B will lead to the gradual failure of liquid A or liquid B. In addition, the contamination of metal ions will reduce the sensitivity of this reagent, please pay attention to the use of clean pipette tips. Store sealed after use. 2. If the background signal is very high after exposure, the possible reason is that the concentration of secondary antibody is too high, or the concentration of primary antibody is too high, or the blocking solution is not suitable. Try reducing the concentration of antibodies, and using other blocking solution that is more compatible with the protein samples. 3. If the fluorescence is quenched quickly, the possible cause is that the fluorescence of the target band is too strong, resulting in rapid consumption of ECL by HRP. 4. If there is no luminescence signal, it may be that the target protein expression is too weak, and longer exposure time may be required. 5. Wear a lab coat and disposable gloves during operation." allowwhitespace="true" highlightstring="" style="box-sizing: inherit; --min-resolution:320; --min-font-size:var(--mobile-base-font-size); --min-font-size-scale:var(--mobile-font-size-scale); --min-fs--2:calc(var(--min-fs--1)/var(--min-font-size-scale)); --min-fs--1:calc(var(--min-fs-0)/var(--min-font-size-scale)); --min-fs-0:var(--min-font-size); --min-fs-1:calc(var(--min-fs-0)*var(--min-font-size-scale)); --min-fs-2:calc(var(--min-fs-1)*var(--min-font-size-scale)); --min-fs-3:calc(var(--min-fs-2)*var(--min-font-size-scale)); --min-fs-4:calc(var(--min-fs-3)*var(--min-font-size-scale)); --min-fs-5:calc(var(--min-fs-4)*var(--min-font-size-scale)); --min-fs-6:calc(var(--min-fs-5)*var(--min-font-size-scale)); --min-fs-7:calc(var(--min-fs-6)*var(--min-font-size-scale)); --max-resolution:1280; --max-font-size:var(--min-font-size); --max-font-size-scale:calc(var(--min-font-size-scale) + 0.11); --max-fs--2:calc(var(--max-fs--1)/var(--max-font-size-scale)); --max-fs--1:calc(var(--max-fs-0)/var(--max-font-size-scale)); --max-fs-0:var(--max-font-size); --max-fs-1:calc(var(--max-fs-0)*var(--max-font-size-scale)); --max-fs-2:calc(var(--max-fs-1)*var(--max-font-size-scale)); --max-fs-3:calc(var(--max-fs-2)*var(--max-font-size-scale)); --max-fs-4:calc(var(--max-fs-3)*var(--max-font-size-scale)); --max-fs-5:calc(var(--max-fs-4)*var(--max-font-size-scale)); --max-fs-6:calc(var(--max-fs-5)*var(--max-font-size-scale)); --max-fs-7:calc(var(--max-fs-6)*var(--max-font-size-scale)); --resolution-range:calc(var(--max-resolution) - var(--min-resolution)); --resolution:1200px; --resolution-progress:calc(var(--resolution) - var(--min-resolution)*1px); --interpolate-by:calc(var(--resolution-progress)/var(--resolution-range)); --range-fs--2:calc(var(--max-fs--2) - var(--min-fs--2)); --range-fs--1:calc(var(--max-fs--1) - var(--min-fs--1)); --range-fs-0:calc(var(--max-fs-0) - var(--min-fs-0)); --range-fs-1:calc(var(--max-fs-1) - var(--min-fs-1)); --range-fs-2:calc(var(--max-fs-2) - var(--min-fs-2)); --range-fs-3:calc(var(--max-fs-3) - var(--min-fs-3)); --range-fs-4:calc(var(--max-fs-4) - var(--min-fs-4)); --range-fs-5:calc(var(--max-fs-5) - var(--min-fs-5)); --range-fs-6:calc(var(--max-fs-6) - var(--min-fs-6)); --range-fs-7:calc(var(--max-fs-7) - var(--min-fs-7)); --fs--2:max(12px,calc(var(--min-fs--2)*1px + var(--range-fs--2)*var(--interpolate-by))); --fs--1:max(14px,calc(var(--min-fs--1)*1px + var(--range-fs--1)*var(--interpolate-by))); --fs-0:calc(var(--min-fs-0)*1px + var(--range-fs-0)*var(--interpolate-by)); --fs-1:calc(var(--min-fs-1)*1px + var(--range-fs-1)*var(--interpolate-by)); --fs-2:calc(var(--min-fs-2)*1px + var(--range-fs-2)*var(--interpolate-by)); --fs-3:calc(var(--min-fs-3)*1px + var(--range-fs-3)*var(--interpolate-by)); --fs-4:calc(var(--min-fs-4)*1px + var(--range-fs-4)*var(--interpolate-by)); --fs-5:calc(var(--min-fs-5)*1px + var(--range-fs-5)*var(--interpolate-by)); --fs-6:calc(var(--min-fs-6)*1px + var(--range-fs-6)*var(--interpolate-by)); --fs-7:calc(var(--min-fs-7)*1px + var(--range-fs-7)*var(--interpolate-by)); --line-height:1.5; --line-height-scale:0.95; --lh--2:calc(var(--lh--1)/var(--line-height-scale)); --lh--1:calc(var(--lh-0)/var(--line-height-scale)); --lh-0:var(--line-height); --lh-1:calc(var(--lh-0)*var(--line-height-scale)); --lh-2:calc(var(--lh-1)*var(--line-height-scale)); --lh-3:calc(var(--lh-2)*var(--line-height-scale)); --lh-4:calc(var(--lh-3)*var(--line-height-scale)); --lh-5:calc(var(--lh-4)*var(--line-height-scale)); --lh-6:calc(var(--lh-5)*var(--line-height-scale)); --lh-7:calc(var(--lh-6)*var(--line-height-scale)); margin: 0px; overflow-wrap: anywhere; word-break: break-word; font-size: var(--fs--1); line-height: var(--lh--1); --mobile-base-font-size:14; --mobile-font-size-scale:1.13;">ECL chemiluminescence Kit is a highly sensitive chemiluminescence kit based on luminol ECL chemiluminescence. This product can react with horseradish peroxidase (HRP) conjugated on secondary antibody to produce fluorescence signal. Samples can be examined by X-ray lithography or other appropriate fluorescence imaging equipment (CCD camera, etc.). This product is further optimized with higher sensitivity and more stable color development, which was suitable for the detection of low abundance target proteins with greater than 2ul of detectable content.Storage and Handling ConditionsWet ice bag transportation; Stored at 4? in the dark; valid for 12 months.Assay Protocol1. Preparation of hypersensitive ECL working solution: Mix the hypersensitive ECL A solution and hypersensitive ECL B solution in equal volumes, and store at 4? away from light. Preparation before use, please use within 2 days.2. In Western blot experiment, after membrane was incubated with secondary antibody, wash several times with 1x PBS, and remove excess liquid. Add the mixed ECL working solution to cover the membrane incubate for 1-2 min.3. Remove the membrane from ECL working liquid with forceps for imaging.Note:1. High sensitive ECL A liquid and high sensitive ECL B liquid in the suction process, the pipette tips must be replaced. The cross contamination of liquid A and liquid B will lead to the gradual failure of liquid A or liquid B. In addition, the contamination of metal ions will reduce the sensitivity of this reagent, please pay attention to the use of clean pipette tips. Store sealed after use.2. If the background signal is very high after exposure, the possible reason is that the concentration of secondary antibody is too high, or the concentration of primary antibody is too high, or the blocking solution is not suitable. Try reducing the concentration of antibodies, and using other blocking solution that is more compatible with the protein samples.3. If the fluorescence is quenched quickly, the possible cause is that the fluorescence of the target band is too strong, resulting in rapid consumption of ECL by HRP.4. If there is no luminescence signal, it may be that the target protein expression is too weak, and longer exposure time may be required.5. Wear a lab coat and disposable gloves during operation.

Price: 111 USD

Location: San Diego, California

End Time: 2025-01-15T00:03:29.000Z

Shipping Cost: 3.99 USD